Food Microbiology Laboratory Plating Techniques ( Dr. Olcay BOYACIOĞLU )
Experiment 1:
Plating techniques
Principles of the Procedure Nutrient Agar consists of peptone, beef extract, and agar. This relatively simple formulation provides the nutrients necessary for the growth of a large number of microorganisms that are not excessively fastidious. The beef extract contains water-soluble substances including carbohydrates, vitamins, organic nitrogen compounds, and salts. Peptones are the principle sources of organic nitrogen, particularly amino acids and long-chained peptides. Agar is the solidifying agent. …