Dersler
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Laboratory > Determination of Proteins v1
A protein in solution can be quantitatively assayed by several spectrophotometric methods. Most methods are based on binding of a chromophore to specific…
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Laboratory > Dialysis
In this experiment we observed the dialysis. The principle of dialysis is that the small molecules can penetrate through the pores of semi-permeable…
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Laboratory > Dialysis v1
Purpose: In this experiment, the dialysis experiment is made. Dialysis is a process that is based on the principle of osmosis, moving from…
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Laboratory > Gel Filtration Using Sephadex
In this experiment we applied gel filtration of enzyme b using sephadex as column material. This is a chromatographic technique used for protein…
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Laboratory > Gel Filtration Using Sephadex v2
When a mixture of protein applied to the top of the gel filtration column, depending on the molecular exclusion limit of column material.…
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Laboratory > Heat Treatment
In this experiment we investigated the effect of temperature on enzyme activity and protein content. After the calculations we plotted two graphs these…
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Laboratory > Heat Treatment v2
In this experiment, Major affect of enzyme activity and stability is temperature. Since enzymes are biochemical catalysts, made up at least partially of…
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Laboratory > Immobilization
Purpose: In this experiment we determined the rate of enzyme activity using free and immobilized enzyme. And our aim in this experiment,…