Genel Mikrobiyoloji
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Rhodophyta ( Laura KELLER )
Rhodophyta Laura Keller What is It? Red algae are marine multicellular organisms that have no cell specialization. In other words, there are no different tissues although red algae are highly branched. They are completely sessile and have no way to move around other than the movement of the water. Most have a reddish or purplish tint, due to the phycobillin pigments they have. Reproductive Cycle Red algae have a very unique reproductive cycle, consisting of two diploid stages and one haploid one. Red algae gametes, just like those of most…
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Mikroskobik Sayım ( MEGEP )
1. BAKTERİ SAYIMI 1.1. Mikroskop Faktörünün Bulunması 1.1.1. Görüş Alanının Çapının Ölçülmesi 1.1.2. Görüş Alanının Hesaplanması 1.1.3. Mikroskop Faktörünün Hesaplanması 1.2. Breed Yöntemi ile Bakteri Sayımı 1.2.1. Amaç ve Prensip 1.2.2. Yapılışı 1.2.3. Hesaplamalar ve Dikkat Edilecek Noktalar 1.2.4. Avantaj ve Dezavantajları 2. MAYA SAYIMI 2.1. Thoma Lamı Yöntemi ile Maya Sayımı 2.1.1. Amaç ve Prensip 2.1.2. Yapılışı 2.2. Hesaplamalar ve Dikkat Edilecek Noktalar 3. KÜFLÜ SAHA SAYIMI 3.1. Howard Lamı Yöntemi ile Küflü Saha Sayımı 3.1.1. Amaç ve Prensip 3.1.2. Yapılışı 3.2. Hesaplamalar ve Dikkat Edilecek Noktalar …
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Gram Stains of Bacteria
Gram Stains of Bacteria Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogenes, Staphylococcus aureus, Staphylococcus epidermis, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Bacillus megaterium, Micrococcus luteus, Pseudomonas aeruginosa, Proteus vulgaris, Mycobacterium smegmatis …
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EMB Agar
EMB agar Only gram-negative bacteria grow on EMB agar. (Gram-positive bacteria are inhibited by the dyes eosin and methylene blue added to the agar.) Based on its rate of lactose fermentation, E. coli produces dark, blue-black colonies with a metallic green sheen on EMB agar. Growing on EMB Agar Nutrient Agar Mannitol Salt Agar Mannitol salt agar is both a selective and differential growth medium. It is used to differentiate pathogenic Staphylococcus species from nonpathogenic members of the genus Micrococcus. Fermentation of mannitol: This medium contains an indicator, phenol…
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Gram Boyama Klinik Kullanım ve Sınırlamalar ( Dr. Özay Arıkan AKAN )
Neden Gram boyama? • Hızlı, ucuz tanı • Etken hakkında fikir verir- ön tanı (Gram reaksiyonu, biçim, büyüklük, morfoloji) • Uygun antibiyotik seçimi • Uygun besiyeri seçimi? • Örnek kalitesini belirler. …
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Enterohemorrhagic Escherichia Coli Infections
In today’s presentation we will cover information regarding Escherichia coli (E. coli) and its epidemiology. We will also talk about the history of the disease, how it is transmitted, species that it affects (including humans), and clinical and necropsy signs observed. Finally, we will address prevention and control measures for E. coli, as well as actions to take if E. coli is suspected. [Photo: Scanning Electron Microscopy (SEM) of Escherichia coli organism. Source: CDC Public Health Image Library] Escherichia coli is a Gram negative rod (bacillus) in the family Enterobacteriaceae.…
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Selective, Differential and Enriched Media
Selective media: used to select (isolate) specific groups of bacteria; chemical substances in the media inhibit the growth of one type of bacteria while permitting growth of another (MSA, EMB, MacConkey) Differential media: distinguishes among morphologically and biochemically related groups of organisms; chemical compounds (following inoculation and incubation) produce a characteristic change in the appearance of bacterial growth and/or the medium surrounding the colonies (MSA, EMB, MacConkey) Enriched media: supplemented with highly nutritious materials, such as blood, serum, or yeast extracts, for the cultivation of fastidious organisms Mannitol Salt Agar…
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Bacterial Staining Endospores
Bacterial Staining Endospores Endospore Structure Function Location Bacteria Endospore Location Simple Stain Endospore Stain Differential stain Endospore vs. Spore Stain 1 = MG with Heat Decolorize = H2O Counterstain = Safranin Results Endospores = Green Vegetative = Pink …
