PURPOSE:

The aim of this experiment is to compare the reaction rate of immobilized enzyme and free enzyme to calculate the effectivness factor

PROCEDURE:

Firstly 1 ml olive oil and 3 ml 25 mM KP buffer ( pH = 7.0 ) were putted into the flask and then 12 mg immobilized enzyme (lipase) was added and mixed. After that it was placed into water bath at 37 ºC for 30 min. After 30 min the flask was centrifuged for 1 min and supernatant that occurs after centrifugation is taken and ethanol was added on it. However, the time passed from starting centrifuge to end of adding ethanol was recorded. And then phenolphthalein was added as indicator and titrated with KOH( 0,05 N). For free enzyme firstly, 1 ml olive oil and 3 ml 25 mM KP buffer ( pH = 7.0 ) were putted into the flask and then 0,1 ml lipase was putted and mixed. After that ıt was placed into water bath at 37 ºC for 30 min. And then phenolphthalein was added as indicator and titrated with KOH. Finally the volume of KOH was recorded.

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