1. The following data were obtained from an enzyme kinetics experiment. Graph the data using a Lineweaver-Burk plot and determine, by inspection of the graph, the values for Km and Vmax.
[S] (µM) V (nmol/min)
_______ ___________
0.20 1.43
0.26 1.67
0.33 2.08
1.00 3.33
2. Calculate the specificity constant for an enzyme if its kcat = 1.4 x 104 s-1 Km = 90 µM. Is this enzyme very efficient?
3. Use the Michaelis-Menton Equation to calculate the missing values of [S] given below if Vmax = 5 mmol/min. Plot [S] versus V (NOT the reciprocals!). Draw line parallel to the x-axis at Vmax and extend your plotted line to show its approach to Vmax.
[S] (mM) V (mmol/min)
_______ ___________
10 1.2
? 1.7
? 2.1
? 2.2
? 2.5
4. The effect of an inhibitor on an enzyme was tested and the experiment gave the results below. Plot the data and determine, by inspection of the graph, what type of inhibition is involved.
[S] µM V (µmol/min) V (µmol/min) V (µmol/min)
with 0.0 nM with 25 nM with 50 nM
Inhibitor Inhibitor Inhibitor
______ ___________ ___________ ___________
0.4 0.22 0.21 0.20
0.67 0.29 0.26 0.24
1.00 0.32 0.30 0.28
2.00 0.40 0.36 0.32
5. You perform a kinetics experiment on the enzyme phosphatidylinositol synthase (PI synthase) using as substrate radiolabeled inositol in a tracer amount mixed with unlabeled inositol
Using a scintillation counter, you have determined that the inositol substrate has a specific radioactivity of 100 dpm (disintegrations per minute) / 1 nmol of inositol.
You collect the following data that represent the amount of radiolabeled phosphatidylinositol formed (data in dpm) after a 10 min reaction using 5 µM enzyme.
[S] nmol/100 µl Reaction Volume dpm Recovered in Product Formed
